A rapid and sensitive p-benzoquinone-mediated bioassay for determination of heavy metal toxicity in water

Abstract

Determining and monitoring toxicity of chemicals in water are very important for human health and country security. Electrochemical measurement of respiratory chain activity is a rapid and reliable screening of the toxicity towards microorganisms. Here, we report a rapid and sensitive toxicity bioassay using p-benzoquinone as the artificial electron mediator and Escherichia coli as the test organism. Four heavy metal ions, Cu²⁺, Ag⁺, Hg²⁺ and Co²⁺, are tested as the model toxicants, and the corresponding 50% respiration inhibition concentrations (IC₅₀) are determined to be 0.95, 8.14, 11.69 and 42.76 mg L⁻¹, respectively. Based on the IC₅₀ values, the descending order of toxicity is: Cu²⁺ > Ag⁺ > Hg²⁺ > Co²⁺. The presented bioassay not only provides a good foundation for further toxicity tests using E. coli, but also the potential for expanding the technique to utilize other bacteria with complementary toxicity responses, thereby allowing use of the bioassay in a wide range of applications.