Issue 6, 2013

Chemiluminescence assay for the sensitive detection of iodide based on extracting Hg2+ from a T–Hg2+–T complex

Abstract

A simple and sensitive chemiluminescence assay for iodide (I) detection was reported, which was based on iodide extracting Hg2+ from DNA featuring a stem-loop structure containing T–Hg2+–T. Specifically, Hg2+ induced random coiled G-rich single-strand DNA to form a stem-loop structure containing T–Hg2+–T. Because the binding of Hg2+ and I is much stronger than that of Hg2+ and thymine (T), I could extract Hg2+ from the stem-loop structure, releasing the DNA, which then bound with K+ and transformed into a K+-stabilized G-quadruplex (with hemin as a cofactor), which catalyzed the H2O2-mediated oxidation of luminol. The produced chemiluminescence as a sensing signal was applied to sensitively and selectively detect iodide with a detection limit of 12 nM. This system exhibited the first DNAzyme-based iodide sensor. Finally, the sensor was successfully applied for iodide detection in real lake water samples.

Graphical abstract: Chemiluminescence assay for the sensitive detection of iodide based on extracting Hg2+ from a T–Hg2+–T complex

Supplementary files

Article information

Article type
Paper
Submitted
13 Nov 2012
Accepted
24 Jan 2013
First published
24 Jan 2013

Analyst, 2013,138, 1898-1902

Chemiluminescence assay for the sensitive detection of iodide based on extracting Hg2+ from a T–Hg2+–T complex

T. Li, G. Liang and X. Li, Analyst, 2013, 138, 1898 DOI: 10.1039/C3AN36673A

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