Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 7, 2013
Previous Article Next Article

Site-directed antibody immobilization using a protein A–gold binding domain fusion protein for enhanced SPR immunosensing

Author affiliations

Abstract

We have implemented a novel strategy for the oriented immobilization of antibodies onto a gold surface based on the use of a fusion protein, the protein A–gold binding domain (PAG). PAG consists of a gold binding peptide (GBP) coupled to the immunoglobulin-binding domains of staphylococcal protein A. This fusion protein provides an easy and fast oriented immobilization of antibodies preserving its native structure, while leaving the antigen binding sites (Fab) freely exposed. Using this immobilization strategy, we have demonstrated the performance of the immunosensing of the human Growth Hormone by SPR. A limit of detection of 90 ng mL−1 was obtained with an inter-chip variability lower than 7%. The comparison of this method with other strategies for the direct immobilization of antibodies over gold surfaces has showed the enhanced sensitivity provided by the PAG approach.

Graphical abstract: Site-directed antibody immobilization using a protein A–gold binding domain fusion protein for enhanced SPR immunosensing

Back to tab navigation

Article information


Submitted
16 Oct 2012
Accepted
22 Jan 2013
First published
23 Jan 2013

Analyst, 2013,138, 2023-2031
Article type
Paper

Site-directed antibody immobilization using a protein A–gold binding domain fusion protein for enhanced SPR immunosensing

E. de Juan-Franco, A. Caruz, J. R. Pedrajas and L. M. Lechuga, Analyst, 2013, 138, 2023
DOI: 10.1039/C3AN36498D

Social activity

Search articles by author

Spotlight

Advertisements