Highly sensitive and selective detection of silver(i) in aqueous solution with silver(i)-specific DNA and Sybr green I

Abstract

We report a label-free fluorescence turn-on approach for the sensitive and selective sensing of silver ion Ag⁺ based on Ag⁺ induced conformational change of cytosine-rich single-stranded DNA. A silver specific oligonucleotide (SSO) undergoes a structural transition from single-stranded DNA (ssDNA) to a C–Ag⁺–C mediated hairpin structure, which can be recognized by a double-stranded DNA (dsDNA) intercalator Sybr Green I (SG). The linear response range for Ag⁺ detection was from 1 nM to 100 nM. The method presented here is highly sensitive and a limit of detection of 1 nM was obtained. More importantly, this optical method was successfully used to identify complex sample mixtures.