Issue 6, 2013
From the journal:

Analyst

Realization of on-tissue protein identification by highly efficient in situ digestion with graphene-immobilized trypsin for MALDI imaging analysis†

Jing Jiao,‡a   Aizhu Miao,‡b   Xinyan Zhang,b   Yan Cai,a   Yi Lu,b   Ying Zhang*a  and  Haojie Lu*a  

* Corresponding authors

a Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, China
E-mail: luhaojie@fudan.edu.cn, ying@fudan.edu.cn

b Department of Ophthalmology, Eye & ENT Hospital of Fudan University, Shanghai, China

Abstract

A novel implementation of in situ protein digestion supported by a graphene oxide-immobilized enzyme reactor (GO-IMER) in the MALDI imaging mass spectrometry (IMS) workflow is reported, which enables the simultaneous diagnostic identity and distribution attributes of the proteome on tissue.

Graphical abstract: Realization of on-tissue protein identification by highly efficient in situ digestion with graphene-immobilized trypsin for MALDI imaging analysis

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Article information

DOI
https://doi.org/10.1039/C3AN36391K
Article type
Communication
Submitted
26 Sep 2012
Accepted
22 Jan 2013
First published
22 Jan 2013

Analyst, 2013,138, 1645-1648

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Realization of on-tissue protein identification by highly efficient in situ digestion with graphene-immobilized trypsin for MALDI imaging analysis

J. Jiao, A. Miao, X. Zhang, Y. Cai, Y. Lu, Y. Zhang and H. Lu, Analyst, 2013, 138, 1645 DOI: 10.1039/C3AN36391K

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