Issue 19, 2013
From the journal:

Analyst

Functionalized viral nanoparticles as ultrasensitive reporters in lateral-flow assays†

Meena Adhikari,‡a   Sagar Dhamane,‡a   Anna E. V. Hagström,b   Gavin Garvey,b   Wen-Hsiang Chen,b   Katerina Kourentzi,b   Ulrich Strycha  and  Richard C. Willson*abc  

* Corresponding authors

a Department of Biology and Biochemistry, University of Houston, Houston, TX 77204-5001, USA

b Department of Chemical and Biomolecular Engineering, University of Houston, Houston, TX 77204-4004, USA

c Houston Methodist Research Institute, Houston, TX 77030, USA
E-mail: willson@uh.edu
Tel: +1 713-743-4308

Abstract

Two types of viral nanoparticles were functionalized with target-specific antibodies and multiple copies of an enzymatic reporter (horseradish peroxidase). The particles were successfully integrated into an immunochromatographic assay detecting MS2 bacteriophage, a model for viral pathogens. The sensitivity of the assay was greatly superior to conventional gold nanoparticle lateral flow assays, and results could easily be evaluated, even without advanced lab instruments.

Graphical abstract: Functionalized viral nanoparticles as ultrasensitive reporters in lateral-flow assays

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Article information

DOI
https://doi.org/10.1039/C3AN00891F
Article type
Communication
Submitted
01 May 2013
Accepted
23 Jul 2013
First published
25 Jul 2013
This article is Open Access
Creative Commons BY license

Analyst, 2013,138, 5584-5587

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Functionalized viral nanoparticles as ultrasensitive reporters in lateral-flow assays

M. Adhikari, S. Dhamane, A. E. V. Hagström, G. Garvey, W. Chen, K. Kourentzi, U. Strych and R. C. Willson, Analyst, 2013, 138, 5584 DOI: 10.1039/C3AN00891F

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