Preconcentration determination of arsenic species by sorption of As(v) on Amberlite IRA-410 coupled with fluorescence quenching of l-cysteine capped CdS nanoparticles

Abstract

A simple and accurate method for arsenic speciation analysis in natural and drinking water samples is described in which preconcentration of arsenic as As(V) was coupled with spectrofluorometric determination. The extracted As(V) species with a column containing Amberlite IRA-410 were subjected to L-cysteine capped CdS quantum dots (QDs) and the fluorescence quenching of the QDs due to reduction of As(V) by L-cysteine was considered as a signal relevant to As(V) concentration. The As(III) species were also determined after oxidation of As(III) ions to As(V) with H₂O₂ and measurement of the total arsenic content. In treatment with 400 mL portions of water samples containing 30 μg L⁻¹ As(V), the relative standard deviation was 2.8%. The detection limit of arsenic was also found to be 0.75 μg L⁻¹ (1 × 10⁻⁸ M). The reliability of proposed method was confirmed using certified reference materials. The trace amounts of arsenic species were then determined in different water samples, satisfactorily.