A highly selective fluorescent probe for BO3− based on acetate derivatives of coumarin in aqueous solution and thimerosal

Abstract

The acetate derivatives of coumarin exhibited a prominent turn-on type signaling behavior toward BO₃⁻ ions over other common anions. Signaling is based on the selective deprotection of acetate groups by perborate, which resulted in significant fluorogenic signaling in an acetate buffered solution (pH 5.0). Interestingly, the detection process makes the raw material of 7-hydroxycoumarin regenerate, and the probe could be applied for the detection of BO₃⁻ of thimerosal. Furthermore, the optical properties of the probe and its BO₃⁻-induced product were theoretically studied based on density functional theory (DFT) and the time-dependent DFT (TDDFT) explored at the B3LYP/6-311+G (d, p) level.