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Issue 1, 2012
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Transducing methyltransferase activity into electrical signals in a carbon nanotube–DNA device

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Abstract

This study creates a device where the DNA is electronically integrated to serve as both the biological target and electrical transducer in a CNT–DNA–CNT device. We detect DNA binding and methylation by the methyltransferase M.SssI at the single molecule level. We demonstrate sequence-specific, reversible binding of M.SssI and protein-catalyzed methylation that alters the protein-binding affinity of the device. This device, which relies on the exquisite electrical sensitivity of DNA, represents a unique route for the specific, single molecule detection of enzymatic activity.

Graphical abstract: Transducing methyltransferase activity into electrical signals in a carbon nanotube–DNA device

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Article information


Submitted
07 Oct 2011
Accepted
19 Oct 2011
First published
20 Oct 2011

Chem. Sci., 2012,3, 62-65
Article type
Edge Article

Transducing methyltransferase activity into electrical signals in a carbon nanotube–DNA device

H. Wang, N. B. Muren, D. Ordinario, A. A. Gorodetsky, J. K. Barton and C. Nuckolls, Chem. Sci., 2012, 3, 62
DOI: 10.1039/C1SC00772F

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