Issue 9, 2012

Tuning the bactericidal repertoire and potency of quinoline-based amphiphiles for enhanced killing of pathogenic bacteria

Abstract

The overwhelming challenge posed by drug-resistant pathogenic bacteria underscores the need for potent bactericidal agents, which exhibit broad-spectrum activity and a mode of action that does not favor development of resistance. In the present study we report the synthesis and bactericidal activity of structurally diverse quinoline-based amphiphiles, having a fluorescent head group and varying hydrophobic chain length. A structure-guided bactericidal efficacy and broad-spectrum activity of the amphiphiles was apparent in screening experiments against a panel of common pathogenic bacteria. Structure–function studies by fluorescence-based assays revealed that the charge and hydrophobic chain length of amphiphiles were key structural determinants that radically boosted the bactericidal activity. The most potent amphiphile N-methyl 8-dodecoxy quinolinium iodide (compound 6) exhibited a dose-dependent bactericidal activity on target pathogens and could even inhibit the growth of a presumptive methicillin-resistant S. aureus (MRSA) strain. Fluorescence-based mechanistic studies and transmission electron microscope (TEM) analysis indicated that the initial binding of compound 6 to bacteria probably involved electrostatic interaction, whereas the hydrophobic chain of the amphiphile promoted membrane insertion, which culminated in large scale membrane disruption and loss in cell viability. Although the bactericidal activity of compound 6 was independent of bacterial transmembrane potential, interaction of the amphiphile with pathogenic bacteria resulted in rapid dissipation of membrane potential. Interestingly, compound 6 displayed high antimicrobial selectivity and did not affect the viability of human HT-29 cells. It is envisaged that the therapeutic regime of the bactericidal scaffold of compound 6 can be further expanded by rational structural design for generating potent bactericidal agents.

Graphical abstract: Tuning the bactericidal repertoire and potency of quinoline-based amphiphiles for enhanced killing of pathogenic bacteria

Supplementary files

Article information

Article type
Paper
Submitted
24 Jan 2012
Accepted
13 Feb 2012
First published
09 Mar 2012

RSC Adv., 2012,2, 3864-3871

Tuning the bactericidal repertoire and potency of quinoline-based amphiphiles for enhanced killing of pathogenic bacteria

U. Vudumula, M. D. Adhikari, B. Ojha, S. Goswami, G. Das and A. Ramesh, RSC Adv., 2012, 2, 3864 DOI: 10.1039/C2RA20140B

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