Issue 10, 2012
From the journal:

RSC Advances

Biocentri-voltammetry for the enzyme assay: a model study

Serdar Çevik,a   Suna Timurb  and  Ülkü Anik*a  

* Corresponding authors

a Mugla University, Faculty of Science, Chemistry Department, Kotekli, Mugla, Turkey
E-mail: ulkuanik@yahoo.com, ulkuanik@gmail.com
Tel: +90 252 211 1503

b Ege University, Faculty of Science, Biochemistry Department, Bornova, Izmir, Turkey.

Abstract

This work constitutes the first biocentri-voltammetric application for acetylcholinesterase (AChE) activity assay. After the optimization of working conditions like reaction time, centrifugation speed and time and enzyme amount, the analytical characteristics were investigated. As a result, the linear range of 1.0–350 μM was obtained with an equation of y = 0.0004x + 0.0163. The correlation coefficient is equal to (R2) 0.99 ± 0.001815 (n = 3) and the RSD value was calculated as 3.6% (n = 4). Then Donepezil-based drug analysis was also conducted by using the developed method and an inhibition of 80% was calculated.

Graphical abstract: Biocentri-voltammetry for the enzyme assay: a model study

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Article information

DOI
https://doi.org/10.1039/C2RA01282K
Article type
Paper
Submitted
12 Dec 2011
Accepted
29 Feb 2012
First published
02 Mar 2012

RSC Adv., 2012,2, 4299-4303

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Biocentri-voltammetry for the enzyme assay: a model study

S. Çevik, S. Timur and Ü. Anik, RSC Adv., 2012, 2, 4299 DOI: 10.1039/C2RA01282K

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