Issue 1, 2012
From the journal:

RSC Advances

Analytical-scale purification of trichostatin A from bacterial culture in a single step and with high selectivity using immobilised metal affinity chromatography

Najwa Ejje,a   Ernest Laceyb  and  Rachel Codd*a  

* Corresponding authors

a School of Medical Sciences (Pharmacology) and Bosch Institute, The University of Sydney, New South Wales, Australia
E-mail: rachel.codd@sydney.edu.au
Fax: 61 2 9351 4717
Tel: 61 2 9351 6738

b Microbial Screening Technologies, Building A, 28-54 Percival Road, Smithfield, New South Wales, Australia
Fax: 61 2 9757 2586
Tel: 61 2 9757 4515

Abstract

The potent histone deacetylase inhibitor trichostatin A (TSA) has been captured in a single step with high selectivity from the culture supernatant of the native TSA-producing strain Streptomyces hygroscopicusMST-AS5346 using analytical-scale Ni(II)-based immobilised metal affinity chromatography.

Graphical abstract: Analytical-scale purification of trichostatin A from bacterial culture in a single step and with high selectivity using immobilised metal affinity chromatography

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Article information

DOI
https://doi.org/10.1039/C1RA00864A
Article type
Paper
Submitted
10 Oct 2011
Accepted
12 Oct 2011
First published
08 Nov 2011

RSC Adv., 2012,2, 333-337

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Analytical-scale purification of trichostatin A from bacterial culture in a single step and with high selectivity using immobilised metal affinity chromatography

N. Ejje, E. Lacey and R. Codd, RSC Adv., 2012, 2, 333 DOI: 10.1039/C1RA00864A

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