Issue 3, 2012
From the journal:

Photochemical & Photobiological Sciences

Caged glucosamine-6-phosphate for the light-control of riboswitch activity†‡

Bernhard Wulffen,a   Maximilian Clemens Rudolf Buff,b   Monika Pofahl,a   Günter Mayer*a  and  Alexander Heckel*b  

* Corresponding authors

a University of Bonn, Life and Medical Sciences, c/o Kekulé-Institute for Organic Chemistry and Biochemistry, Gerhard-Domagk-Str. 1, Bonn, Germany
E-mail: gmayer@uni-bonn.de
Fax: +49 (228) 73-4809
Tel: +49 (228) 73-4808

b Goethe University Frankfurt, Frankfurt Institute for Molecular Life Sciences, Max-von-Laue-Str. 9, Frankfurt (M), Germany
E-mail: heckel@uni-frankfurt.de
Fax: +49 (69) 798-763 42505
Tel: +49 (69) 798-42505

Abstract

We have synthesized a light-activatable (“caged”) derivative of glucosamine-6-phosphate (GlcN6P), which only upon irradiation becomes a cofactor for the glmS riboswitch. This glmS riboswitch maintains its activity when embedded in the 3′-untranslated region of eukaryotic mRNA molecules and caged GlcN6P reduces the amount of translated EGFP upon irradiation with light in vitro.

Graphical abstract: Caged glucosamine-6-phosphate for the light-control of riboswitch activity

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Article information

DOI
https://doi.org/10.1039/C1PP05242J
Article type
Communication
Submitted
05 Aug 2011
Accepted
22 Sep 2011
First published
17 Oct 2011

Photochem. Photobiol. Sci., 2012,11, 489-492

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Caged glucosamine-6-phosphate for the light-control of riboswitch activity

B. Wulffen, M. C. R. Buff, M. Pofahl, G. Mayer and A. Heckel, Photochem. Photobiol. Sci., 2012, 11, 489 DOI: 10.1039/C1PP05242J

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