Issue 6, 2012
From the journal:

Photochemical & Photobiological Sciences

CdSe/ZnS core shell quantum dot-based FRET binary oligonucleotide probes for detection of nucleic acids†

Yiru Peng,‡ac   Chunmei Qiu,‡b   Steffen Jockusch,a   Amy M. Scott,a   Zengmin Li,b   Nicholas J. Turro*a  and  Jingyue Ju*b  

* Corresponding authors

a Department of Chemistry, Columbia University, New York, NY, USA

b Department of Chemical Engineering, Columbia University, New York, NY, USA

c College of Chemistry & Material Sciences, Fujian Normal University, Fuzhou, China

Abstract

We report the design, synthesis, and characterization of a binary oligonucleotide probe for selective DNA or RNA detection. The probe is based on fluorescence resonance energy transfer (FRET) from quantum dot (CdSe/ZnS core shell) DNA conjugates to organic dye (cyanine-5) DNA conjugates. Selective hybridization of the donor/acceptor DNA conjugates to target DNA enhances FRET and a change in fluorescence signature was observed.

Graphical abstract: CdSe/ZnS core shell quantum dot-based FRET binary oligonucleotide probes for detection of nucleic acids

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Article information

DOI
https://doi.org/10.1039/C1PP05132F
Article type
Communication
Submitted
05 May 2011
Accepted
09 Aug 2011
First published
26 Aug 2011

Photochem. Photobiol. Sci., 2012,11, 881-884

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CdSe/ZnS core shell quantum dot-based FRET binary oligonucleotide probes for detection of nucleic acids

Y. Peng, C. Qiu, S. Jockusch, A. M. Scott, Z. Li, N. J. Turro and J. Ju, Photochem. Photobiol. Sci., 2012, 11, 881 DOI: 10.1039/C1PP05132F

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