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Issue 44, 2012
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Synthesis of 2-arylindole derivatives and evaluation as nitric oxide synthase and NFκB inhibitors

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Abstract

Development of small molecule drug-like inhibitors blocking both nitric oxide synthase and NFκB could offer a synergistic therapeutic approach in the prevention and treatment of inflammation and cancer. During the course of evaluating the biological potential of a commercial compound library, 2-phenylindole (1) displayed inhibitory activity against nitrite production and NFκB with IC50 values of 38.1 ± 1.8 and 25.4 ± 2.1 μM, respectively. Based on this lead, synthesis and systematic optimization have been undertaken in an effort to find novel and more potent nitric oxide synthase and NFκB inhibitors with antiinflammatory and cancer preventive potential. First, chemical derivatizations of 1 and 2-phenylindole-3-carboxaldehyde (4) were performed to generate a panel of N-alkylated indoles and 3-oxime derivatives 2–7. Second, a series of diversified 2-arylindole derivatives (10) were synthesized from an array of substituted 2-iodoanilines (8) and terminal alkynes (9) by applying a one-pot palladium catalyzed Sonogashira-type alkynylation and base-assisted cycloaddition. Subsequent biological evaluations revealed 3-carboxaldehyde oxime and cyano substituted 2-phenylindoles 5 and 7 exhibited the strongest nitrite inhibitory activities (IC50 = 4.4 ± 0.5 and 4.8 ± 0.4 μM, respectively); as well as NFκB inhibition (IC50 = 6.9 ± 0.8 and 8.5 ± 2.0 μM, respectively). In addition, the 6′-MeO-naphthalen-2′-yl indole derivative 10at displayed excellent inhibitory activity against NFκB with an IC50 value of 0.6 ± 0.2 μM.

Graphical abstract: Synthesis of 2-arylindole derivatives and evaluation as nitric oxide synthase and NFκB inhibitors

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Supplementary files

Article information


Submitted
26 Jul 2012
Accepted
13 Sep 2012
First published
13 Sep 2012

Org. Biomol. Chem., 2012,10, 8835-8847
Article type
Paper

Synthesis of 2-arylindole derivatives and evaluation as nitric oxide synthase and NFκB inhibitors

X. Yu, E. Park, T. P. Kondratyuk, J. M. Pezzuto and D. Sun, Org. Biomol. Chem., 2012, 10, 8835
DOI: 10.1039/C2OB26456K

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