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Issue 31, 2012
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Discovery, application and protein engineering of Baeyer–Villiger monooxygenases for organic synthesis

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Abstract

Baeyer–Villiger monooxygenases (BVMOs) are useful enzymes for organic synthesis as they enable the direct and highly regio- and stereoselective oxidation of ketones to esters or lactones simply with molecular oxygen. This contribution covers novel concepts such as searching in protein sequence databases using distinct motifs to discover new Baeyer–Villiger monooxygenases as well as high-throughput assays to facilitate protein engineering in order to improve BVMOs with respect to substrate range, enantioselectivity, thermostability and other properties. Recent examples for the application of BVMOs in synthetic organic synthesis illustrate the broad potential of these biocatalysts. Furthermore, methods to facilitate the more efficient use of BVMOs in organic synthesis by applying e.g. improved cofactor regeneration, substrate feed and in situ product removal or immobilization are covered in this perspective.

Graphical abstract: Discovery, application and protein engineering of Baeyer–Villiger monooxygenases for organic synthesis

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Publication details

The article was received on 10 Apr 2012, accepted on 08 Jun 2012 and first published on 13 Jun 2012


Article type: Perspective
DOI: 10.1039/C2OB25704A
Org. Biomol. Chem., 2012,10, 6249-6265

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    Discovery, application and protein engineering of Baeyer–Villiger monooxygenases for organic synthesis

    K. Balke, M. Kadow, H. Mallin, S. Saß and U. T. Bornscheuer, Org. Biomol. Chem., 2012, 10, 6249
    DOI: 10.1039/C2OB25704A

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