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Issue 7, 2012
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Determination of surface concentrations of individual molecule-layers used in nanoscale biosensors by in situATR-FTIR spectroscopy

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Abstract

For the development of nanowire sensors for chemical and medical detection purposes, the optimal functionalization of the surface is a mandatory component. Quantitative ATR-FTIR spectroscopy was used in situ to investigate the step-by-step layer formation of typical functionalization protocols and to determine the respective molecule surface concentrations. BSA, anti-TNF-α and anti-PSA antibodies were bound via 3-(trimethoxy)butylsilyl aldehyde linkers to silicon-oxide surfaces in order to investigate surface functionalization of nanowires. Maximum determined surface concentrations were 7.17 × 10−13 mol cm−2 for BSA, 1.7 × 10−13 mol cm−2 for anti-TNF-α antibody, 6.1 × 10−13 mol cm−2 for anti-PSA antibody, 3.88 × 10−13 mol cm−2 for TNF-α and 7.0 × 10−13 mol cm−2 for PSA. Furthermore we performed antibody–antigen binding experiments and determined the specific binding ratios. The maximum possible ratio of 2 was obtained at bulk concentrations of the antigen in the μg ml−1 range for TNF-α and PSA.

Graphical abstract: Determination of surface concentrations of individual molecule-layers used in nanoscale biosensors by in situ ATR-FTIR spectroscopy

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Article information


Submitted
20 Dec 2011
Accepted
02 Feb 2012
First published
03 Feb 2012

Nanoscale, 2012,4, 2431-2438
Article type
Paper

Determination of surface concentrations of individual molecule-layers used in nanoscale biosensors by in situ ATR-FTIR spectroscopy

M. Punzet, D. Baurecht, F. Varga, H. Karlic and C. Heitzinger, Nanoscale, 2012, 4, 2431
DOI: 10.1039/C2NR12038K

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