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Issue 4, 2012
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Improving metallomics information related to transgenic and non-transgenic soybean seeds using 2D-HPLC-ICP-MS and ESI-MS/MS

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Abstract

This work reports the use of 2D-HPLC-ICP-MS to enlarge metallomics information when considering soybean seeds. Separations using size exclusion chromatography (SEC) allowed the identification of three metal fractions: the first corresponding to molecular weights from 38.1 to 181.1 kDa, the second from 8.2 to 17.2 kDa and the third from 0.4 to 3.8 kDa. In a second dimension, using anion exchange chromatography (AEX), three sub-fractions containing Fe, Mg and Mn, one containing Cu, and three containing Co, Cu, Mg, Mn and Zn were obtained. After these separations, 33 proteins were identified using the ESI-MS/MS technique, and divided into four functional categories: plant growth/cell division, protein destination and storage, metabolism and unclassified proteins. Among the identified proteins, proteins previously related to metals were found.

Graphical abstract: Improving metallomics information related to transgenic and non-transgenic soybean seeds using 2D-HPLC-ICP-MS and ESI-MS/MS

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Publication details

The article was received on 20 Dec 2011, accepted on 13 Feb 2012 and first published on 14 Feb 2012


Article type: Paper
DOI: 10.1039/C2MT00186A
Metallomics, 2012,4, 373-378

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    Improving metallomics information related to transgenic and non-transgenic soybean seeds using 2D-HPLC-ICP-MS and ESI-MS/MS

    L. R. Verola Mataveli, M. Fioramonte, F. C. Gozzo and M. A. Zezzi Arruda, Metallomics, 2012, 4, 373
    DOI: 10.1039/C2MT00186A

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