Structural and thermodynamic characterization of the self-adhesive properties of human P-cadherin

Shota Kudo; Jose M. M. Caaveiro; Takamitsu Miyafusa; Shuichiro Goda; Keisuke Ishii; Tadashi Matsuura; Yukio Sudou; Tatsuhiko Kodama; Takao Hamakubo; Kouhei Tsumoto

doi:10.1039/C2MB25161B

Structural and thermodynamic characterization of the self-adhesive properties of human P-cadherin†

Shota Kudo,^a Jose M. M. Caaveiro,^b Takamitsu Miyafusa,^a Shuichiro Goda,^c Keisuke Ishii,^d Tadashi Matsuura,^d Yukio Sudou,^d Tatsuhiko Kodama,^e Takao Hamakubo^e and Kouhei Tsumoto*^ab

Author affiliations

* Corresponding authors

^a Department of Medical Genome Sciences, The University of Tokyo, Tokyo, Japan
E-mail: tsumoto@ims.u-tokyo.ac.jp
Fax: +81 3-6409-2129
Tel: +81 3-5449-5316

^b Institute of Medical Sciences, The University of Tokyo, Tokyo, Japan

^c Faculty of Engineering, Nagasaki University, Nagasaki, Japan

^d Perseus Proteomics Inc., Komaba, Tokyo, Japan

^e Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo, Japan

Abstract

Human P-cadherin is a promising therapeutic target against cancer. However, its characterization at the molecular level is still lacking. We report that human P-cadherin associated irreversibly in a distinct dimer configuration. Unexpectedly, the divalent cation Ca²⁺ was not necessary for dimerization, although it greatly stabilized the protein–protein complex.

Molecular BioSystems

Structural and thermodynamic characterization of the self-adhesive properties of human P-cadherin†

Abstract

Supplementary files

Article information

Download Citation

Permissions

Structural and thermodynamic characterization of the self-adhesive properties of human P-cadherin

Search articles by author

Spotlight

Advertisements