Issue 2, 2012
From the journal:

Molecular BioSystems

Specific and reversible photochemical labeling of plasmid DNA using photoresponsive oligonucleotides containing 3-cyanovinylcarbazole†

Kenzo Fujimoto,*ab   Kaoru Hiratsuka-Konishi,a   Takashi Sakamoto,a   Tomoko Ohtake,a   Ken-ichi Shinoharaa  and  Yoshinaga Yoshimuraa  

* Corresponding authors

a School of Materials Science, Japan Advanced institute of Science and Technology, 1–1 Asahi-dai, Nomi, Japan
E-mail: kenzo@jaist.ac.jp
Fax: +81 761 51 1671
Tel: +81 761 51 1671

b Research Center for Bio-Architecture, Japan Advanced institute of Science and Technology, 1–1 Asahi-dai, Nomi, Japan
E-mail: kenzo@jaist.ac.jp
Fax: +81 761 51 1671
Tel: +81 761 51 1671

Abstract

To develop a covalent and specific labeling method for single- and double-stranded plasmid DNA, photoresponsive oligonucleotide containing 3-cyanovinylcarbazole nucleoside was adopted. Single- and double-stranded plasmid DNA was successfully labeled/de-labeled with Cy3 and/or biotin by photoirradiation.

Graphical abstract: Specific and reversible photochemical labeling of plasmid DNA using photoresponsive oligonucleotides containing 3-cyanovinylcarbazole

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Article information

DOI
https://doi.org/10.1039/C2MB05422A
Article type
Communication
Submitted
12 Oct 2011
Accepted
30 Nov 2011
First published
19 Dec 2011

Mol. BioSyst., 2012,8, 491-494

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Specific and reversible photochemical labeling of plasmid DNA using photoresponsive oligonucleotides containing 3-cyanovinylcarbazole

K. Fujimoto, K. Hiratsuka-Konishi, T. Sakamoto, T. Ohtake, K. Shinohara and Y. Yoshimura, Mol. BioSyst., 2012, 8, 491 DOI: 10.1039/C2MB05422A

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