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Issue 3, 2012
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Human in vivo longevity is reflected in vitro by differential metabolism as measured by 1H-NMR profiling of cell culture supernatants

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Abstract

The offspring of nonagenarian siblings suffer less from age related conditions and have a lower risk of mortality compared to their partners. Fibroblast strains derived from such offspring in middle age show different in vitro responses to stress, more stress-induced apoptosis and less senescence when compared to strains of their partners. Aiming to find differences in cellular metabolismin vitro between these fibroblast strains, cell culture supernatants collected at 24 hours and five days were analysed using 1H nuclear magnetic resonance (NMR)-based metabolic footprinting. Between 24 hours and five days of incubation, supernatants of all fibroblast strains showed decreased levels of glucose, pyruvate, alanineglutamine (ala-gln), valine, leucine, isoleucine, serine and lysine and increased levels of glutamine, alanine, lactate and pyroglutamic acid. Strains from offspring and their partners were compared using a partial least squares-discriminant analysis (PLS-DA) model based on the data of the five-day time point. The ala-gln and glucose consumption were higher for fibroblast strains derived from offspring when compared to strains of their partners. Also, production of glutamine, alanine, lactate and pyroglutamic acid was found to be higher for fibroblast strains derived from offspring. In conclusion, differences in NMR-based metabolic profiles of human cellsin vitro reflect the propensity for human longevity of the subjects from whom these were derived.

Graphical abstract: Human in vivo longevity is reflected in vitro by differential metabolism as measured by 1H-NMR profiling of cell culture supernatants

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Supplementary files

Article information


Submitted
15 Jun 2011
Accepted
22 Nov 2011
First published
04 Jan 2012

Mol. BioSyst., 2012,8, 783-789
Article type
Paper

Human in vivo longevity is reflected in vitro by differential metabolism as measured by 1H-NMR profiling of cell culture supernatants

P. Dekker, A. Meissner, R. W. Dirks, P. Eline Slagboom, D. van Heemst, A. M. Deelder, H. J. Tanke, R. G. J. Westendorp and A. B. Maier, Mol. BioSyst., 2012, 8, 783
DOI: 10.1039/C2MB05237G

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