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Issue 20, 2012
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Automated generation of libraries of nL droplets

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Abstract

We demonstrate an integrated system for rapid and automated generation of multiple, chemically distinct populations of ∼103–104 sub-nanoliter droplets. Generation of these ‘libraries of droplets’ proceeds in the following automated steps: i) generation of a sequence of micro-liter droplets of individually predetermined composition, ii) injection of these ‘parental’ droplets onto a chip, iii) transition from a mm- to a μm-scale of the channels and splitting each of the parental drops with a flow-focusing module into thousands of tightly monodisperse daughter drops and iv) separation of such formed homogeneous populations with plugs of a third immiscible fluid. This method is compatible both with aspiration of microliter portions of liquid from a 96-well plate with a robotic station and with automated microfluidic systems that generate (∼μL) droplets of preprogrammed compositions. The system that we present bridges the techniques that provide elasticity of protocols executed on microliter droplets with the techniques for high-throughput screening of small (∼pL, ∼nL) droplet libraries. The method that we describe can be useful in exploiting the synergy between the ability to rapidly screen distinct chemical environments and to perform high-throughput studies of single cells or molecules and in digital droplet PCR systems.

Graphical abstract: Automated generation of libraries of nL droplets

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Publication details

The article was received on 10 May 2012, accepted on 23 Aug 2012 and first published on 24 Aug 2012


Article type: Paper
DOI: 10.1039/C2LC40540G
Citation: Lab Chip, 2012,12, 3995-4002
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    Automated generation of libraries of nL droplets

    T. S. Kaminski, S. Jakiela, M. A. Czekalska, W. Postek and P. Garstecki, Lab Chip, 2012, 12, 3995
    DOI: 10.1039/C2LC40540G

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