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Issue 18, 2012
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Denaturation mapping of Saccharomyces cerevisiae

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Abstract

Optical mapping of DNA provides large-scale genomic information that can be used to assemble contigs from next-generation sequencing, and to detect rearrangements between single cells. A recent optical mapping technique called denaturation mapping has the advantage of using physical principles rather than the action of enzymes to probe genomic structure. Denaturation mapping uses fluorescence microscopy to image the pattern of partial melting along a DNA molecule extended in a channel of cross-section 120 nm at the heart of a nanofluidic device. We used denaturation mapping to locate single DNA molecules on the yeast genome (12.1 Mbp) by comparing images to a computationally predicted map for the entire genome sequence. By locating 84 molecules we assembled an optical map of the yeast genome with > 50% coverage.

Graphical abstract: Denaturation mapping of Saccharomyces cerevisiae

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Article information


Submitted
03 May 2012
Accepted
20 Jun 2012
First published
23 Jul 2012

Lab Chip, 2012,12, 3314-3321
Article type
Paper

Denaturation mapping of Saccharomyces cerevisiae

R. L. Welch, R. Sladek, K. Dewar and W. W. Reisner, Lab Chip, 2012, 12, 3314
DOI: 10.1039/C2LC40504K

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