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Issue 20, 2012
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Real-time full-spectral imaging and affinity measurements from 50 microfluidic channels using nanohole surface plasmon resonance

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Abstract

With recent advances in high-throughput proteomics and systems biology, there is a growing demand for new instruments that can precisely quantify a wide range of receptor–ligand binding kinetics in a high-throughput fashion. Here we demonstrate a surface plasmon resonance (SPR) imaging spectroscopy instrument capable of simultaneously extracting binding kinetics and affinities from 50 parallel microfluidic channels. The instrument utilizes large-area (∼ cm2) metallic nanohole arrays as SPR sensing substrates and combines a broadband light source, a high-resolution imaging spectrometer and a low-noise CCD camera to extract spectral information from every channel in real time with a refractive index resolution of 7.7 × 10−6 refractive index units. To demonstrate the utility of our instrument for quantifying a wide range of biomolecular interactions, each parallel microfluidic channel is coated with a biomimetic supported lipid membrane containing ganglioside (GM1) receptors. The binding kinetics of cholera toxin b (CTX-b) to GM1 are then measured in a single experiment from 50 channels. By combining the highly parallel microfluidic device with large-area periodic nanohole array chips, our SPR imaging spectrometer system enables high-throughput, label-free, real-time SPR biosensing, and its full-spectral imaging capability combined with nanohole arrays could enable integration of SPR imaging with concurrent surface-enhanced Raman spectroscopy.

Graphical abstract: Real-time full-spectral imaging and affinity measurements from 50 microfluidic channels using nanohole surface plasmon resonance

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Article information


Submitted
30 Apr 2012
Accepted
21 Jun 2012
First published
26 Jun 2012

Lab Chip, 2012,12, 3882-3890
Article type
Paper

Real-time full-spectral imaging and affinity measurements from 50 microfluidic channels using nanohole surface plasmon resonance

S. H. Lee, N. C. Lindquist, N. J. Wittenberg, L. R. Jordan and S. Oh, Lab Chip, 2012, 12, 3882
DOI: 10.1039/C2LC40455A

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