Jump to main content
Jump to site search

Issue 15, 2012
Previous Article Next Article

Proteolysis in microfluidic droplets: an approach to interface protein separation and peptide mass spectrometry

Author affiliations

Abstract

A versatile microreactor protocol based on microfluidic droplets has been developed for on-line protein digestion. Proteins separated by liquid chromatography are fractionated in water-in-oil droplets and digested in sequence. The microfluidic reactor acts also as an electrospray ionization emitter for mass spectrometry analysis of the peptides produced in the individual droplets. Each droplet is an enzymatic micro-reaction unit with efficient proteolysis due to rapid mixing, enhanced mass transfer and automated handling. This droplet approach eliminates sample loss, cross-contamination, non-specific absorption and memory effect. A protein mixture was successfully identified using the droplet-based micro-reactor as interface between reverse phase liquid chromatography and mass spectrometry.

Graphical abstract: Proteolysis in microfluidic droplets: an approach to interface protein separation and peptide mass spectrometry

Back to tab navigation

Supplementary files

Publication details

The article was received on 25 Feb 2012, accepted on 01 May 2012 and first published on 02 May 2012


Article type: Communication
DOI: 10.1039/C2LC40206H
Citation: Lab Chip, 2012,12, 2625-2629
  •   Request permissions

    Proteolysis in microfluidic droplets: an approach to interface protein separation and peptide mass spectrometry

    J. Ji, L. Nie, L. Qiao, Y. Li, L. Guo, B. Liu, P. Yang and H. H. Girault, Lab Chip, 2012, 12, 2625
    DOI: 10.1039/C2LC40206H

Search articles by author

Spotlight

Advertisements