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Issue 7, 2012
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Concentration landscape generators for shear free dynamic chemical stimulation

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Abstract

In this paper we first introduce a novel fabrication process, which allows for easy integration of thin track-etched nanoporous membranes, within 2D or 3D microchannel networks. In these networks, soluble chemical compounds can diffuse out of the channels through well-defined and spatially organized microfabricated porous openings. Interestingly, multiple micron-scale porous areas can be integrated in the same device and each of these areas can be connected to a different microfluidic channel and reservoir. We then present and characterize several membrane-based microdevices and their use for the generation of stable diffusible concentration gradients and complex dynamic chemical landscapes under shear free conditions. We also demonstrate how a simple flow-focusing geometry can be used to generate “on-demand” concentration profiles. In turn, these devices should provide an ideal experimental framework for high throughput cell-based assays: long term high-resolution video microscopy experiments can be performed, under multiple spatially and temporally controlled chemical conditions, with simple protocols and in a cell-friendly environment.

Graphical abstract: Concentration landscape generators for shear free dynamic chemical stimulation

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Publication details

The article was received on 14 Oct 2011, accepted on 10 Jan 2012 and first published on 20 Feb 2012


Article type: Paper
DOI: 10.1039/C2LC20994B
Citation: Lab Chip, 2012,12, 1340-1346

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    Concentration landscape generators for shear free dynamic chemical stimulation

    M. Morel, J. Galas, M. Dahan and V. Studer, Lab Chip, 2012, 12, 1340
    DOI: 10.1039/C2LC20994B

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