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Issue 5, 2012
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A 3D mammalian cell separator biochip

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Abstract

The dissimilar cytoskeletal architecture in diverse cell types induces a difference in their deformability that presents a viable approach to separate cells in a non-invasive manner. We report on the design and fabrication of a robust and scalable device capable of separating a heterogeneous population of cells with variable degree of deformability into enriched populations with deformability above a certain threshold. The three dimensional device was fabricated in fused silica by femtosecond laser direct writing combined with selective chemical etching. The separator device was evaluated using promyelocytic HL60 cells. Using flow rates as large as 167 μL min−1, throughputs of up to 2800 cells min−1 were achieved at the device output. A fluorescence-activated cell sorting (FACS) viability analysis on the cells revealed 81% of the population maintain cellular integrity after passage through the device.

Graphical abstract: A 3D mammalian cell separator biochip

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Publication details

The article was received on 27 Sep 2011, accepted on 03 Jan 2012 and first published on 18 Jan 2012


Article type: Paper
DOI: 10.1039/C2LC20939J
Citation: Lab Chip, 2012,12, 948-953

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    A 3D mammalian cell separator biochip

    D. Choudhury, W. T. Ramsay, R. Kiss, N. A. Willoughby, L. Paterson and A. K. Kar, Lab Chip, 2012, 12, 948
    DOI: 10.1039/C2LC20939J

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