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Issue 3, 2012
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Microfluidics with aqueous two-phase systems

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An overview is given about research activities in which aqueous two phase systems (ATPSs) are utilized in microfluidic setups. ATPSs consist of two immiscible aqueous phases and have traditionally been used for the separation and purification of biological material such as proteins or cells. Microfluidic implementations of such schemes are usually based on a number of co-flowing streams of immiscible phases in a microchannel, thereby replacing the standard batch by flow-through processes. Some aspects of the stability of such flow patterns and the recovery of the phases at the channel exit are reviewed. Furthermore, the diffusive mass transfer and sample partitioning between the phases are discussed, and corresponding applications are highlighted. When diffusion is superposed by an applied electric field normal to the liquid/liquid interface, the transport processes are accelerated, and under specific conditions the interface acts as a size-selective filter for molecules. Finally, the activities involving droplet microflows of ATPSs are reviewed. By either forming ATPS droplets in an organic phase or a droplet of one aqueous phase inside the other, a range of applications has been demonstrated, extending from separation/purification schemes to the patterning of surfaces covered with cells.

Graphical abstract: Microfluidics with aqueous two-phase systems

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Publication details

The article was received on 28 Jun 2011, accepted on 10 Aug 2011 and first published on 07 Sep 2011

Article type: Tutorial Review
DOI: 10.1039/C1LC20569B
Lab Chip, 2012,12, 434-442

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    Microfluidics with aqueous two-phase systems

    S. Hardt and T. Hahn, Lab Chip, 2012, 12, 434
    DOI: 10.1039/C1LC20569B

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