Issue 7, 2012
From the journal:

Lab on a Chip

Pulsed laser triggered high speed microfluidic fluorescence activated cell sorter†‡

Ting-Hsiang Wu,ab   Yue Chen,a   Sung-Yong Park,a   Jason Hong,b   Tara Teslaa,b   Jiang F. Zhong,c   Dino Di Carlo,d   Michael A. Teitellb  and  Pei-Yu Chiou*a  

* Corresponding authors

a Department of Mechanical and Aerospace Engineering, University of California, Los Angeles (UCLA), 420 Westwood Plaza, Rm 37-138, Los Angeles, CA, USA
E-mail: pychiou@seas.ucla.edu
Tel: +1-310-825-8620

b Department of Pathology and Laboratory Medicine, UCLA, Los Angeles, CA, USA
E-mail: mteitell@mednet.ucla.edu
Fax: +1-310- 267-0382
Tel: +1- 310- 206-6754

c Department of Biochemistry and Molecular Biology, University of Southern California, 2011 Zonal Avenue, HMR 308, Los Angeles, CA, USA
E-mail: jzhong@usc.edu
Tel: +1- 323- 442-2371

d Department of Bioengineering, UCLA, 420 Westwood Plaza Rm 5121 ENG V, Los Angeles, CA, USA
E-mail: dicarlo@seas.ucla.edu
Tel: +1-310- 983-3235

Abstract

We report a high speed and high purity pulsed laser triggered fluorescence activated cell sorter (PLACS) with a sorting throughput up to 20 000 mammalian cells s−1 with 37% sorting purity, 90% cell viability in enrichment mode, and >90% purity in high purity mode at 1500 cells s−1 or 3000 beads s−1. Fast switching (30 μs) and a small perturbation volume (∼90 pL) is achieved by a unique sorting mechanism in which explosive vapor bubbles are generated using focused laser pulses in a single layer microfluidic PDMS channel.

Graphical abstract: Pulsed laser triggered high speed microfluidic fluorescence activated cell sorter

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Article information

DOI
https://doi.org/10.1039/C2LC21084C
Article type
Paper
Submitted
08 Nov 2011
Accepted
09 Feb 2012
First published
15 Feb 2012

Lab Chip, 2012,12, 1378-1383

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Pulsed laser triggered high speed microfluidic fluorescence activated cell sorter

T. Wu, Y. Chen, S. Park, J. Hong, T. Teslaa, J. F. Zhong, D. Di Carlo, M. A. Teitell and P. Chiou, Lab Chip, 2012, 12, 1378 DOI: 10.1039/C2LC21084C

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