Issue 4, 2012
From the journal:

Lab on a Chip

Single-vesicle estimation of ATP-binding cassette transporters in microfluidic channels†

Hirotaka Sasaki,a   Ryuji Kawano,a   Toshihisa Osaki,a   Koki Kamiyaa  and  Shoji Takeuchi*ab  

* Corresponding authors

a Kanagawa Academy of Science and Technology, 3-2-1 Sakado, Takatsu-ku, Kawasaki, Japan

b Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, Japan
E-mail: takeuchi@iis.u-tokyo.ac.jp
Fax: +81 (3) 5452 6649
Tel: +81 (3) 5452 6650

Abstract

We have developed a method to analyze the substrate transport of ATP-binding cassette (ABC) transporters, which are associated with drug resistance in tumor cells. Our microfluidic method is well suited to the single-vesicle estimation of substrate transport and the rapid drug screening of ABC transporters. Using this method, we have demonstrated, for the first time, the analysis of substrate transport by a single transporter and performed drug-inhibition experiments in less than 3 h.

Graphical abstract: Single-vesicle estimation of ATP-binding cassette transporters in microfluidic channels

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Article information

DOI
https://doi.org/10.1039/C2LC21058D
Article type
Communication
Submitted
01 Nov 2011
Accepted
30 Nov 2011
First published
30 Nov 2011

Lab Chip, 2012,12, 702-704

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Single-vesicle estimation of ATP-binding cassette transporters in microfluidic channels

H. Sasaki, R. Kawano, T. Osaki, K. Kamiya and S. Takeuchi, Lab Chip, 2012, 12, 702 DOI: 10.1039/C2LC21058D

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