Issue 1, 2012

Enhancement of static incubation time in microfluidic cell culture platforms exploiting extended air–liquid interface

Abstract

Microfluidics based cell culture applications have facilitated the study of cellular dynamics at the single entity level. Yet, long term versions of such applications in a static framework suffer from the fast exhaustion of available oxygen, dissolved in the limited media volume available per cell, within the microconfined environment. In order to circumvent such drawbacks, we have improvised a microfluidic cell culture platform for prolonged sustenance of adherent mammalian cells by formation of an air–liquid interface through functionalizing inner surfaces of a polydimethylsiloxane (PDMS) based microdevice. We have demonstrated an augmented static incubation time for different cell lines using this approach.

Graphical abstract: Enhancement of static incubation time in microfluidic cell culture platforms exploiting extended air–liquid interface

Supplementary files

Article information

Article type
Communication
Submitted
15 Sep 2011
Accepted
12 Oct 2011
First published
10 Nov 2011

Lab Chip, 2012,12, 69-73

Enhancement of static incubation time in microfluidic cell culture platforms exploiting extended air–liquid interface

N. Bose, T. Das, D. Chakraborty, T. K. Maiti and S. Chakraborty, Lab Chip, 2012, 12, 69 DOI: 10.1039/C1LC20888H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements