Issue 3, 2012

Label-free real-time imaging in microchip free-flow electrophoresis applying high speed deep UV fluorescence scanning

Abstract

We report on label-free monitoring of microfluidic free-flow electrophoresis (μFFE) separations in real-time using a custom built high speed deep UV laser scanner. In combination with a novel layout realized in fused silica (FS) FFE chips the setup was successfully applied for continuous separations and detection of unlabeled analytes including native proteins by space-resolved intrinsic deep UV fluorescence scanning.

Graphical abstract: Label-free real-time imaging in microchip free-flow electrophoresis applying high speed deep UV fluorescence scanning

Article information

Article type
Paper
Submitted
27 Jun 2011
Accepted
19 Sep 2011
First published
19 Oct 2011

Lab Chip, 2012,12, 458-463

Label-free real-time imaging in microchip free-flow electrophoresis applying high speed deep UV fluorescence scanning

S. Köhler, S. Nagl, S. Fritzsche and D. Belder, Lab Chip, 2012, 12, 458 DOI: 10.1039/C1LC20558G

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