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Issue 39, 2012
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Antibody-functionalized polymer-coated gold nanoparticles targeting cancer cells: an in vitro and in vivo study

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Abstract

Gold nanoparticles (∼5 nm) coated with plasma-polymerized allylamine were produced through plasma vapor deposition and bioconjugated with a monoclonal antibody targeting the epidermal growth factor receptor. The resulting nanoconjugates displayed an antibody loading of about 1.7 nmol mg−1 and efficiently target epidermal growth factor receptor overexpressing cell lines, as ascertained by ELISA and Western blot assays. The in vitro targeting properties were also confirmed in vivo, where a similar biodistribution profile of what was experienced for the unconjugated antibody was observed. Thanks to the possibility of doping the gold nanoparticles with radionuclides during plasma vapor deposition, the proposed functionalization strategy represents a very suitable platform for the in vivo cancer targeting with nanosized multifunctional particles.

Graphical abstract: Antibody-functionalized polymer-coated gold nanoparticles targeting cancer cells: an in vitro and in vivo study

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Article information


Submitted
30 May 2012
Accepted
29 Jul 2012
First published
02 Aug 2012

J. Mater. Chem., 2012,22, 21305-21312
Article type
Paper

Antibody-functionalized polymer-coated gold nanoparticles targeting cancer cells: an in vitro and in vivo study

R. Marega, L. Karmani, L. Flamant, P. G. Nageswaran, V. Valembois, B. Masereel, O. Feron, T. V. Borght, S. Lucas, C. Michiels, B. Gallez and D. Bonifazi, J. Mater. Chem., 2012, 22, 21305
DOI: 10.1039/C2JM33482H

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