Jump to main content
Jump to site search

Issue 15, 2012
Previous Article Next Article

Sulfonic groups induce formation of filopodia in mesenchymal stem cells

Author affiliations

Abstract

Glycosaminoglycans (GAGs) are an integral part of the extracellular matrix and glycocalix, i.e. the closest cellular environment. They are abundant in –OH groups and their bioactivity is also associated with the presence of negatively charged –SO3H functionalities. Therefore, we have investigated and discussed the influence of these functional units on mesenchymal stem cells (MSCs) behaviour using single component and mixed self-assembled monolayers of alkanethiols with –SO3H and –OH end groups. In the absence of serum, MSCs attachment, spreading, cytoskeleton organisation and motility were significantly influenced by the surface chemistry. We found that the sulfonic groups induce star-like cell shape with very intense actin staining and a high density net of filopodia that enlarge from the base of lamellipodia structures. Moreover, this response is concentration dependent and is apparent only for very short culture time in the presence of serum.

Graphical abstract: Sulfonic groups induce formation of filopodia in mesenchymal stem cells

Back to tab navigation

Supplementary files

Article information


Submitted
09 Nov 2011
Accepted
31 Jan 2012
First published
02 Mar 2012

J. Mater. Chem., 2012,22, 7172-7178
Article type
Paper

Sulfonic groups induce formation of filopodia in mesenchymal stem cells

D. S. da Costa, R. A. Pires, A. M. Frias, R. L. Reis and I. Pashkuleva, J. Mater. Chem., 2012, 22, 7172
DOI: 10.1039/C2JM15762D

Search articles by author

Spotlight

Advertisements