Preparation and application of lysozyme imprinted monolithic column with dopamine as the functional monomer

Abstract

A novel protein imprinted monolithic column, which combines the merits of high selectivity of a molecular imprinting column and the new functional monomer dopamine, was prepared for selective separation of the template protein lysozyme (Lyz). The macroporous poly(GMA-DVB) skeleton was synthesized in a 4.6 mm i.d. stainless steel column with glycidyl methacrylate (GMA) and divinylbenzene (DVB) as precursors. Subsequently, the molecularly imprinted polymer (MIP) film coating with dopamine as the functional monomer and Lyz as imprinted template was noncovalently attached to the organic skeleton. The produced Lyz-MIP monoliths were characterized using high performance liquid chromatography (HPLC) analysis and exhibited excellent chromatographic performance and recognition ability. The imprinted factor (IF) reached 5.04. Furthermore, the MIP monolith was applied to separate the Lyz from chicken egg white with satisfactory results.