Issue 8, 2012

Carbonic anhydrase assisted calcium carbonate crystalline composites as a biocatalyst

Abstract

In the present study, we report on the carbonic anhydrase (CA)-assisted formation of biomineralized calcium carbonate crystalline composites (CCCCs). Ellipsoidal CCCCs, such as calcite polymorphism, in a micro-size range catalyzed by CA were successfully synthesized with polyethylene glycol and magnetic nanoparticles in the constant CO2 pressure controlled chamber, for the first time. CA-assisted CCCCs characterized by X-ray diffraction, transmission electron microscopy, scanning electron microscopy, and dynamic light scattering, showed their crystalline phase with mesoporous property according to Fourier transform infrared and Brunauer–Emmett–Teller area. These CCCCs retained about 43% of free CA esterase activity. Furthermore, the magnet-based separation was also successful for the reuse of the CCCCs. As a result, the CCCCs produced preserved their catalytic activity even after its ten repeated usages, and were stable for more than 50 days under room temperature. The reported method paves the way for novel biomineralization via CA for the formation of functional CA containing nanocomposites and biocatalyst technology applications.

Graphical abstract: Carbonic anhydrase assisted calcium carbonate crystalline composites as a biocatalyst

Article information

Article type
Paper
Submitted
25 Mar 2012
Accepted
15 May 2012
First published
15 May 2012

Green Chem., 2012,14, 2216-2220

Carbonic anhydrase assisted calcium carbonate crystalline composites as a biocatalyst

E. T. Hwang, H. Gang, J. Chung and M. B. Gu, Green Chem., 2012, 14, 2216 DOI: 10.1039/C2GC35444F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements