Issue 3, 2012
From the journal:

Catalysis Science & Technology

A nanocluster design for the construction of artificial cellulosomes†

Do-Myoung Kim,a   Hikaru Nakazawa,a   Mitsuo Umetsu,*a   Takashi Matsuyama,b   Nobuhiro Ishida,b   Akinori Ikeuchi,b   Haruo Takahashi,b   Ryutaro Asanoa  and  Izumi Kumagaia  

* Corresponding authors

a Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba 6-6-11, Aramaki, Aoba-ku, Sendai, Japan
E-mail: mitsuo@kuma.che.tohoku.ac.jp
Fax: +81-22-795-7276
Tel: +81-22-795-7276

b Toyota Central R&D Lab, Yokomichi 41-1, Oaza Nagakute, Nagakute-cho, Aichi-gun, Japan

Abstract

We describe here the construction of artificial cellulosomes by nanoclustering recombinant cellulolytic modules on non-cellulosome-derived scaffolds. Catalytic and cellulose-binding domain modules derived from cellulosomes were assembled on streptavidin and on inorganic nanoparticles. Heteroclustering of the modules significantly promoted the activity of the assembled catalytic modules for degradation of water-insoluble substrates.

Graphical abstract: A nanocluster design for the construction of artificial cellulosomes

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Article information

DOI
https://doi.org/10.1039/C2CY00371F
Article type
Communication
Submitted
15 Sep 2011
Accepted
27 Dec 2011
First published
13 Jan 2012

Catal. Sci. Technol., 2012,2, 499-503

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A nanocluster design for the construction of artificial cellulosomes

D. Kim, H. Nakazawa, M. Umetsu, T. Matsuyama, N. Ishida, A. Ikeuchi, H. Takahashi, R. Asano and I. Kumagai, Catal. Sci. Technol., 2012, 2, 499 DOI: 10.1039/C2CY00371F

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