Different strategies to enhance the activity of lipase catalysts

Abstract

The design of new strategies for improving the catalytic activity of lipases in their application on non-natural substrates is in great demand due to their biotechnological applications. This perspective illustrates the power of technologies, such as the immobilization of enzymes on nanomaterials and the site-specific chemical modification of enzyme surfaces, to prepare highly active semisynthetic lipases. These techniques have produced optimal biocatalysts for different important bioprocesses such as biodiesel production, regioselective deprotection of carbohydrates and kinetic resolution of different drug precursors.