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Issue 26, 2012
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Coherent manipulation of free amino acids fluorescence

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Coherent manipulation of molecular wavepackets in biomolecules might contribute to the quest towards label-free cellular imaging and protein identification. We report the use of optimally tailored UV laser pulses in pump–probe depletion experiments that selectively enhance or decrease fluorescence between two aromatic amino acids: tryptophan (Trp) and tyrosine (Tyr). Selective fluorescence modulation is achieved with a contrast of ∼35%. A neat modification of the time-dependent fluorescence depletion signal of Trp is observed, while the Tyr transient trace remains unchanged. The mechanism invoked for explaining the change of the depletion of Trp is a less efficient coupling between the fluorescing state and the higher non-radiative excited states by the optimally shaped pulse, than by the reference pulse.

Graphical abstract: Coherent manipulation of free amino acids fluorescence

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Article information

24 Oct 2011
16 Feb 2012
First published
06 Mar 2012

Phys. Chem. Chem. Phys., 2012,14, 9317-9322
Article type

Coherent manipulation of free amino acids fluorescence

A. Rondi, L. Bonacina, A. Trisorio, C. Hauri and J.-P. Wolf, Phys. Chem. Chem. Phys., 2012, 14, 9317
DOI: 10.1039/C2CP23357F

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