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Issue 89, 2012
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A modified guanosine phosphoramidite for click functionalization of RNA on the sugar edge§

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Abstract

A propargyl containing guanosine phosphoramidite was synthesized and incorporated into siRNA, enabling click-ligation with an azido fluorophore onto the nucleobase sugar edge. Duplex stability was not affected by labeling at this new site, which allowed deconvolution of the effects of label, structure and attachment site on RNAi activity.

Graphical abstract: A modified guanosine phosphoramidite for click functionalization of RNA on the sugar edge

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Supplementary files

Article information


Submitted
05 Jun 2012
Accepted
17 Sep 2012
First published
18 Sep 2012

This article is Open Access

Chem. Commun., 2012,48, 11014-11016
Article type
Communication

A modified guanosine phosphoramidite for click functionalization of RNA on the sugar edge

S. Seidu-Larry, B. Krieg, M. Hirsch, M. Helm and O. Domingo, Chem. Commun., 2012, 48, 11014
DOI: 10.1039/C2CC34015A

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