Jump to main content
Jump to site search

Issue 21, 2012
Previous Article Next Article

Efficient one-pot multienzyme synthesis of UDP-sugars using a promiscuous UDP-sugar pyrophosphorylase from Bifidobacterium longum (BLUSP)

Author affiliations

Abstract

A promiscuous UDP-sugar pyrophosphorylase (BLUSP) was cloned from Bifidobacterium longum strain ATCC55813 and used efficiently with a Pasteurella multocida inorganic pyrophosphatase (PmPpA) with or without a monosaccharide 1-kinase for one-pot multienzyme synthesis of UDP-galactose, UDP-glucose, UDP-mannose, and their derivatives. Further chemical diversification of a UDP-mannose derivative resulted in the formation of UDP-N-acetylmannosamine.

Graphical abstract: Efficient one-pot multienzyme synthesis of UDP-sugars using a promiscuous UDP-sugar pyrophosphorylase from Bifidobacterium longum (BLUSP)

Back to tab navigation

Supplementary files

Publication details

The article was received on 05 Dec 2011, accepted on 09 Jan 2012 and first published on 10 Jan 2012


Article type: Communication
DOI: 10.1039/C2CC17577K
Chem. Commun., 2012,48, 2728-2730

  •   Request permissions

    Efficient one-pot multienzyme synthesis of UDP-sugars using a promiscuous UDP-sugar pyrophosphorylase from Bifidobacterium longum (BLUSP)

    M. M. Muthana, J. Qu, Y. Li, L. Zhang, H. Yu, L. Ding, H. Malekan and X. Chen, Chem. Commun., 2012, 48, 2728
    DOI: 10.1039/C2CC17577K

Search articles by author

Spotlight

Advertisements