Issue 96, 2012

Atomic force microscopy captures folded ribosome bound nascent chains

Abstract

Direct visualization of co-translational folding of nascent polypeptide chains is challenging. Here we present, for the first time, AFM images of large protein constructs based on the membrane binding domain of ankyrin-R, complexed with the ribosome. The characteristic “horse-shoe” shape of ankyrin-R emerging from the ribosome was captured.

Graphical abstract: Atomic force microscopy captures folded ribosome bound nascent chains

Supplementary files

Article information

Article type
Communication
Submitted
31 Jul 2012
Accepted
25 Sep 2012
First published
26 Sep 2012

Chem. Commun., 2012,48, 11727-11729

Atomic force microscopy captures folded ribosome bound nascent chains

A. Loksztejn, Z. Scholl and P. E. Marszalek, Chem. Commun., 2012, 48, 11727 DOI: 10.1039/C2CC35551E

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