Issue 65, 2012

Templated chemistry for monitoring damage and repair directly in duplex DNA§

Abstract

We report the fluorogenic detection of the product of base excision repair (an abasic site) in a specific sequence of duplex DNA. This is achieved by DNA-templated chemistry, employing triple helix-forming probes that contain unnatural nucleobases designed to selectively recognize the site of a missing base. Light-up signals of up to 36-fold were documented, and probes could be used to monitor enzymatic removal of a damaged base.

Graphical abstract: Templated chemistry for monitoring damage and repair directly in duplex DNA

Supplementary files

Article information

Article type
Communication
Submitted
06 Jun 2012
Accepted
27 Jun 2012
First published
28 Jun 2012

Chem. Commun., 2012,48, 8069-8071

Templated chemistry for monitoring damage and repair directly in duplex DNA

S. H. Lee, S. Wang and E. T. Kool, Chem. Commun., 2012, 48, 8069 DOI: 10.1039/C2CC34060G

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