Issue 68, 2012
From the journal:

Chemical Communications

Time-gated luminescence microscopy with responsive nonmetal probes for mapping activity of protein kinases in living cells†

Angela Vaasa,a   Kadri Ligi,a   Shabnam Mohandessi,b   Erki Enkvist,a   Asko Uri*a  and  Lawrence W. Millerb  

* Corresponding authors

a Institute of Chemistry, University of Tartu, 14A Ravila St., 50411 Tartu, Estonia
E-mail: asko.uri@ut.ee
Fax: +372 737 5275
Tel: +372 737 5275

b Department of Chemistry, University of Illinois at Chicago, 845 W. Taylor St., Chicago, USA

Abstract

A photoluminescence probe ARC-1185, possessing both high affinity towards basophilic protein kinases (PKs) and microsecond-scale luminescence lifetime when associated with a kinase, was used for the mapping of ARC-1185–PK complexes in living cells with time-gated luminescence microscopy.

Graphical abstract: Time-gated luminescence microscopy with responsive nonmetal probes for mapping activity of protein kinases in living cells

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C2CC33565D
Article type
Communication
Submitted
17 May 2012
Accepted
02 Jul 2012
First published
03 Jul 2012

Chem. Commun., 2012,48, 8595-8597

Permissions

Request permissions

Time-gated luminescence microscopy with responsive nonmetal probes for mapping activity of protein kinases in living cells

A. Vaasa, K. Ligi, S. Mohandessi, E. Enkvist, A. Uri and L. W. Miller, Chem. Commun., 2012, 48, 8595 DOI: 10.1039/C2CC33565D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements