Issue 48, 2012
From the journal:

Chemical Communications

A sensitive and reliable detection of thrombin via enzyme-precipitate-coating-linked aptamer assay†

Hye-Jin Lee,a   Byoung Chan Kim,b   Min-Kyu Oh*a  and  Jungbae Kim*a  

* Corresponding authors

a Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, Republic of Korea
E-mail: mkoh@korea.ac.kr, jbkim3@korea.ac.kr
Fax: +82-2-926-6102
Tel: +82-2-3290-4850

b Center for Environment, Health and Welfare Research, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea

Abstract

Stable enzyme precipitate coatings (EPCs) with high enzyme loading were employed to develop a sensitive and reliable detection protocol, termed EPC-linked aptamer assay (EPC-LAA). EPC-LAA achieved the limit of detection to be 0.5 ng per mL of thrombin (13.5 pM), and maintained a reasonably stable detection signal even after storing all the reagents at 40 °C for 66 days.

Graphical abstract: A sensitive and reliable detection of thrombin via enzyme-precipitate-coating-linked aptamer assay

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Article information

DOI
https://doi.org/10.1039/C2CC30710C
Article type
Communication
Submitted
01 Feb 2012
Accepted
04 Apr 2012
First published
05 Apr 2012

Chem. Commun., 2012,48, 5971-5973

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A sensitive and reliable detection of thrombin via enzyme-precipitate-coating-linked aptamer assay

H. Lee, B. C. Kim, M. Oh and J. Kim, Chem. Commun., 2012, 48, 5971 DOI: 10.1039/C2CC30710C

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