Pyrosequencing-based barcodes for a dye-free multiplex bioassay

Zhiyao Chen; Xiaoying Fu; Xiaodan Zhang; Xiqun Liu; Bingjie Zou; Haiping Wu; Qinxin Song; Jinheng Li; Tomoharu Kajiyama; Hideki Kambara; Guohua Zhou

doi:10.1039/C2CC17618A

Pyrosequencing-based barcodes for a dye-free multiplex bioassay†

Zhiyao Chen,^a Xiaoying Fu,^b Xiaodan Zhang,^b Xiqun Liu,^b Bingjie Zou,^b Haiping Wu,^b Qinxin Song,^a Jinheng Li,^a Tomoharu Kajiyama,^c Hideki Kambara^c and Guohua Zhou*^ab

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^a Department of Pharmacology, Jinling Hospital, Nanjing University School of Medicine, Nanjing, PR China
E-mail: ghzhou@nju.edu.cn
Fax: +86 25 84514223
Tel: +86 25 84514223

^b Huadong Research Institute for Medicine and Biotechnics, Nanjing, PR China

^c Central Research Laboratory, Hitachi, Ltd., 1-280 Higashi-Koigakubo, Kokubunji-shi, Tokyo 185-8601, Japan

Abstract

A novel dye-free labeling method for a multiplex bioassay was proposed by using short sequence-based barcodes consisting of a reporter base and repeats of two stuffer bases; then, the barcodes were quantitatively decoded by a single pyrosequencing assay without any pre-separation.

Chemical Communications

Pyrosequencing-based barcodes for a dye-free multiplex bioassay†

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Pyrosequencing-based barcodes for a dye-free multiplex bioassay

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