Issue 4, 2012
From the journal:

Chemical Communications

Site-selective modification of proteins for the synthesis of structurally defined multivalent scaffolds†‡

Lukas M. Artner,a   Lars Merkel,b   Nina Bohlke,b   Figen Beceren-Braun,c   Christoph Weise,a   Jens Dernedde,c   Nediljko Budisa*b  and  Christian P. R. Hackenberger*a  

* Corresponding authors

a Freie Universität Berlin, Institut für Chemie und Biochemie, Takustr. 3, 14195 Berlin, Germany
E-mail: hackenbe@chemie.fu-berlin.de
Fax: +49 30 838 52551

b TU Berlin, Institut für Chemie, Franklinstraße 29, D-10587 Berlin, Germany
E-mail: budisa@biocat.tu-berlin.de
Fax: +49 30 314 28 279

c Charité—Universitätsmedizin Berlin, Institut für Laboratoriumsmedizin, Klinische Chemie und Pathobiochemie, CBF, Hindenburgdamm 30, 12200 Berlin

Abstract

A combination of classical site-directed mutagenesis, genetic code engineering and bioorthogonal reactions delivered a chemically modified barstar protein with one or four carbohydrates installed at specific residues. These protein conjugates were employed in multivalent binding studies, which support the use of proteins as structurally defined scaffolds for the presentation of multivalent ligands.

Graphical abstract: Site-selective modification of proteins for the synthesis of structurally defined multivalent scaffolds

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Article information

DOI
https://doi.org/10.1039/C1CC16039G
Article type
Communication
Submitted
28 Sep 2011
Accepted
17 Oct 2011
First published
09 Nov 2011

Chem. Commun., 2012,48, 522-524

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Site-selective modification of proteins for the synthesis of structurally defined multivalent scaffolds

L. M. Artner, L. Merkel, N. Bohlke, F. Beceren-Braun, C. Weise, J. Dernedde, N. Budisa and C. P. R. Hackenberger, Chem. Commun., 2012, 48, 522 DOI: 10.1039/C1CC16039G

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