Jump to main content
Jump to site search

Issue 8, 2012
Previous Article Next Article

A capillary electrophoresis method for the simultaneous analysis of ammonium and metals in animal wastes used in biogas production

Author affiliations

Abstract

Capillary electrophoresis (CE) was studied for the simultaneous analysis of ammonium and metals from animal wastes used in biogas production. The feedstock materials for fermentation consisted of dairy manure and a mixture of dairy manure and fish offal waste. An imidazole background electrolyte containing 18-crown-6-ether and 30% methanol was capable of the efficient separation of ammonium, sodium, potassium, magnesium and calcium in the samples. CE results for the analysis of metals (K+, Ca2+, and Mg2+) in animal wastes were comparable to the results obtained with ICP-MS/OES analysis. Furthermore, the CE results of the analysis of ammonium in dairy manure samples were well comparable with the titrimetric/distillation method. Interestingly, the analysis of ammonium in fish waste samples by the titrimetric/distillation or the spectrophotometric method used was not reliable, likely due to matrix interferences. In contrast, the CE analysis of all analytes and matrices was reproducible and matrix interferences were not found. The reproducibility of the CE method calculated as the relative standard deviation was ≤10% both for the intra-day (n ≥ 7) analysis and during a three month inter-day (n = 45) study. The only sample pre-treatment needed was the dilution of water extract prior to the CE analysis. In addition, the separation time in CE analysis was only six minutes. Therefore, capillary electrophoresis can be considered to be a very competitive alternative method for rapid and reliable simultaneous analysis of ammonium and metals in fermentation samples containing animal wastes.

Graphical abstract: A capillary electrophoresis method for the simultaneous analysis of ammonium and metals in animal wastes used in biogas production

Back to tab navigation

Publication details

The article was received on 26 Mar 2012, accepted on 29 May 2012 and first published on 31 May 2012


Article type: Paper
DOI: 10.1039/C2AY25307K
Anal. Methods, 2012,4, 2278-2282

  •   Request permissions

    A capillary electrophoresis method for the simultaneous analysis of ammonium and metals in animal wastes used in biogas production

    M. Jaakkola, M. Lipponen, J. Kallio and V. Virtanen, Anal. Methods, 2012, 4, 2278
    DOI: 10.1039/C2AY25307K

Search articles by author

Spotlight

Advertisements