Jump to main content
Jump to site search

Issue 6, 2012
Previous Article Next Article

Detection and removal of mercury and lead ions by using gold nanoparticle-based gel membrane

Author affiliations

Abstract

This paper describes the use of two types of bovine serum albumin (BSA)-capped 14.2 nm diameter gold nanoparticles (Au NPs) for the separate detection of mercury (Hg2+) and lead (Pb2+) ions in highly saline media. The BSA-capped Au NPs were stable in solutions containing up to 500 mM NaCl. Introduction of BSA to a solution of rhodamine 6G (R6G) and 3-mercaptopropionic acid (MPA)-modified Au NPs (R6G/MPA–Au NPs) provided a R6G/MPA–Au NP@BSA probe for the sensing of Hg2+ ions. We also used BSA-capped Au NPs to detect Pb2+ ions through a mechanism based on Pb2+ ions accelerating the leaching rate of Au NPs in the presence of thiosulfate (S2O32−) and 2-mercaptoethanol (2-ME). The resulting deposition of Hg2+ ions onto the Au NPs induced the release of R6G from the surfaces of the Au NPs, causing increased fluorescence from the R6G/MPA–Au NP@BSA solution. The Pb2+ ions accelerated the dissolution of the 2-ME/S2O32−–Au NPs@BSA into solution, leading to dramatic decreases in the absorption. These two Au NP-based probes were highly sensitive (LOD ≈ nM) and selective (over 100-fold against other metal ions) toward Hg2+ or Pb2+ ions. We validated the practicality of these two probes through analyses of seawater and urine samples. We also developed a simple gel-based membrane for removal and sensing of Hg2+ or Pb2+ in aqueous solutions. The agarose gel was used to trap BSA-Au NPs, leading to the preparation of a nanocomposite film of Au NPs@BSA-decorated agarose gel membrane (Au NPs@BSA/AGM) for removing Hg2+ or Pb2+ in solution. In addition, R6G/MPA–Au NP@BSA-trapped agarose gel membrane (R6G/MPA–Au NP@BSA/AGM) and 2-ME/S2O32−–Au NPs@BSA/AGM allowed for the rapid and simple detection of Hg2+ and Pb2+, respectively.

Graphical abstract: Detection and removal of mercury and lead ions by using gold nanoparticle-based gel membrane

Back to tab navigation

Supplementary files

Publication details

The article was received on 08 Dec 2011, accepted on 21 Mar 2012 and first published on 23 Mar 2012


Article type: Paper
DOI: 10.1039/C2AY05872C
Citation: Anal. Methods, 2012,4, 1709-1717
  •   Request permissions

    Detection and removal of mercury and lead ions by using gold nanoparticle-based gel membrane

    Y. Lee, F. Nan, M. Chen, H. Wu, C. Ho, Y. Chen and C. Huang, Anal. Methods, 2012, 4, 1709
    DOI: 10.1039/C2AY05872C

Search articles by author

Spotlight

Advertisements