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Issue 5, 2012
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Electrochemical behavior and analytical detection of insulin on pretreated nanocarbon black electrode surface

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Abstract

This work takes advantage of electrochemically anodic pretreated carbon paste electrode modified with acetylene black nanocarbon particles (AB/CPE), a new and high-sensitive analytical method for insulin was put forward. Modern voltammetric testing techniques were used for primarily investigating the redox electrochemical characterization of Fe(CN)63−/4− and electrochemical behavior of insulin on nanocarbon electrode surface. At the same time, a plausible mechanism was also proposed to provide insights into understanding how to facilitate the electron transfer between insulin biomolecule and electrode surface. The results have shown that the pretreated AB/CPE represented high accumulation efficiency to insulin and promoted its direct electron transfer rate owing to the presence of nanocarbon particles and anodic pretreatment. It is found that insulin exhibited a very sensitive anodic peak at 0.47 V on the pretreated AB/CPE, and its peak current was increased about six times more than that on the pretreated carbon paste electrode (CPE). A linear relationship between the anodic peak current and the concentration of insulin from 20 to 1000 nM and a limit of detection as low as 5 nM were obtained using the pretreated AB/CPE. Attracting attention, the proposed method was applied to the realistic samples successfully and showed good recovery and reproducibility.

Graphical abstract: Electrochemical behavior and analytical detection of insulin on pretreated nanocarbon black electrode surface

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Publication details

The article was received on 25 Nov 2011, accepted on 06 Mar 2012 and first published on 30 Mar 2012


Article type: Paper
DOI: 10.1039/C2AY05828F
Citation: Anal. Methods, 2012,4, 1377-1382
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    Electrochemical behavior and analytical detection of insulin on pretreated nanocarbon black electrode surface

    C. Guo, C. Chen, Z. Luo and L. Chen, Anal. Methods, 2012, 4, 1377
    DOI: 10.1039/C2AY05828F

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